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KMID : 0880220070450010069
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Journal of Microbiology
2007 Volume.45 No. 1 p.69 ~ p.74
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Genetic Characterization of the Escherichia coli O66 Antigen and Functional Identification of its wzy Gene
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Cheng Jiansong
Liu Bin
Bastin David A.
Han Weiqing
Wang Lei
Feng Lu
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Abstract
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Escherichia coli is a clonal species, and occurs as both commensal and pathogenic strains, which are normally classified on the basis of their O, H, and K antigens. The O-antigen (O-specific polysaccharide), which consists of a series of oligosaccharide (O-unit) repeats, contributes major antigenic variability to the cell surface. The O-antigen gene cluster of E. coli O66 was sequenced in this study. The genes putatively responsible for the biosynthesis of dTDP-6-deoxy-L-talose and GDP-mannose, as well as those responsible for the transfer of sugars and for O-unit processing were identified based on their homology. The function of the wzy gene was confirmed by the results of a mutation test. Genes specific for E. coli O66 were identified via PCR screening against representatives of 186 E. coli and Shigella O type strains. The comparison of intergenic sequences located between galF and the O-antigen gene cluster in a range of E. coli and Shigella showed that this region may perform an important function in the homologous recombination of the O-antigen gene clusters.
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KEYWORD
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Escherichia coli O-antigen gene cluster, PCR typing, molecular evolution, O-antigen polymerase gene (wzy)
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